Methods

20. Dunn, A.R., and Spudich, J.A. (2011). Single-molecule gold-nanoparticle tracking. 2011: 1498-1506. 
(full pdf text)

19. Spudich, J.A., Rice, S.E., Rock, R.S., Purcell, T.J., and Warrick HM. (2011). The optical trapping dumbbell assay for nonprocessive motors or motors that turn around filaments. Cold Spring Harb Protoc.2011: 1372-1374. (full pdf text)

18. Spudich, J.A., Rice, S.E., Rock, R.S., Purcell, T.J., and Warrick, H.M. (2011). Attachment of anti-GFP antibodies to microspheres for optical trapping experiments. Cold Spring Harb Protoc. 2011:1370-1371. 
(full pdf text)

17. Spudich, J.A., Rice, S.E., Rock, R.S., Purcell. T.J., and Warrick, H.M. (2011). Optical traps to study properties of molecular motors. Cold Spring Harb Protoc.2011: 1305-18. (full pdf text)

16. Sung, J.M., Sivaramakrishnan, S., Dunn, A. and Spudich, J.A. (2009). Single Molecule Dual Beam Optical Trap Analyses of Protein Structure and Function. Methods Enzymol. 475: 321-375. (full pdf text)

15. Dunn, A.R.D. and Spudich, J.A. (2008). Single-molecule Gold-nanoparticle Tracking with High Temporal and Spatial Resolution and without an Applied Load. in Laboratory Manual for Single Molecule Studies, P.R. Selvin and T. Ha, eds., Cold Spring Harbor Laboratory Press, pp. 421-430. (full pdf text.)

14. Spudich, J.A., Rice, S.E., Rock, R.S., Purcell, T.J. and Warrick, H.M. (2008). Optical Traps to Study Properties of Molecular Motors. in Laboratory Manual for Single Molecule Studies, P.R. Selvin and T. Ha, eds., Cold Spring Harbor Laboratory Press, pp. 279-296. (full pdf text.)

13. Churchman, L.S. and Spudich, J.A. (2008). Colocalization of Fluorescent Probes: Accurate and Precise Registration with Nanometer Resolution. in Laboratory Manual for Single Molecule Studies, P.R. Selvin and T. Ha, eds., Cold Spring Harbor Laboratory Press, pp. 73-84. (full pdf text)

12. Rice, S.E., Purcell, T.J., and Spudich, J.A. (2003). Building and Using Optical Traps to Study Properties of Molecular Motors. Methods Enzymol. 361: 112-133. (Full PDF text available.)

11. Rock, R.S., Rief, M., Mehta, A.D. and Spudich, J.A. (2000). In Vitro Assays of Processive Myosin Motors. Methods 22: 373-381. (full pdf text)

10. Mehta, A.D., Finer, J.T., and Spudich, J.A. (1998). Use of Optical Traps in Single-Molecule Study of Nonprocessive Biological Motors. Methods in Enzymology 298: 436-459. (full pdf text)

9. Mehta, A.D., Finer, J.T., and Spudich, J.A. (1998). Reflections of a Lucid Dreamer: Optical Trap Design Considerations. in Methods in Cell Biology, Vol 55. M.P. Sheetz, editor (Academic Press), pp. 47-69. 
full pdf text.)

8. Warrick, H.M., Simmons, R.M., Finer, J.T., Uyeda, T.Q.P., Chu, S., and Spudich, J.A. (1993). In Vitro Methods for Measuring Force and Velocity of the Actin-Myosin Interaction using Purified Proteins. inMethods in Cell Biology, Vol. 39, Scholey, J.M. editor (Academic Press), pp. 1-21. (full pdf text)

7. Kron, S.J., Toyoshima, Y.Y., Uyeda, T.Q.P., and Spudich, J.A. (1991). Assays for Actin Sliding Movement over Myosin-Coated Surfaces. Methods in Enzymology 196: 399-416. (full pdf text.)

6. Egelhoff, T.T., Titus, M.A., Manstein, D.J., Ruppel, K.M., and Spudich, J.A. (1991). Molecular Genetic Tools for Study of the Cytoskeleton in Dictyo­stelium. Methods in Enzymology 196: 319-334. (full pdf text)

5. Spudich, J.A. (editor). (1987). Dictyostelium discoideum: Molecular Approaches to Cell Biology. Methods in Cell Biology, Vol. 28. (Four chapters and the Appendix contributed from this laboratory). (full pdf text)

4. Sheetz, M.P., Block, S.M., and Spudich, J.A. (1987). Myosin Movement In Vitro: A Quanti­tative Assay Using Oriented Actin Cables from Nitella. Methods in Enzymology 134: 531-544. (full pdf text.)

3. Pardee, J.D., and Spudich, J.A. (1982). Purification of Muscle Actin. Methods in Enzymology 85: 164-181. (full pdf text)

2. Spudich, J.A. (1982). Dictyostelium discoideum: Methods and Perspectives for Study of Cell Motility.Methods in Cell Biol. 25: 359-364. (full pdf text.)

1. Pardee, J.D., and Spudich, J.A. (1982). Purification of Muscle Actin. Methods in Cell Biol. 24: 271-289. 
(full pdf text